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Biosensors
Volume 14
Issue 6
10.3390/bios14060263
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Open AccessArticle
by Xu Chen SciProfilesScilitPreprints.orgGoogle Scholar Xuhui Huang Xuhui Huang SciProfilesScilitPreprints.orgGoogle Scholar Saima Kanwal Saima Kanwal SciProfilesScilitPreprints.orgGoogle Scholar Jian Wang Jian Wang SciProfilesScilitPreprints.orgGoogle Scholar Jing Wen Jing Wen SciProfilesScilitPreprints.orgGoogle Scholar Dawei Zhang Dawei Zhang SciProfilesScilitPreprints.orgGoogle Scholar
1
School of Optical-Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
2
Engineering Research Center of Optical Instrument and System, The Ministry of Education, Shanghai Key Laboratory of Modern Optical System, University of Shanghai for Science and Technology, Shanghai 200093, China
*
Author to whom correspondence should be addressed.
Biosensors 2024, 14(6), 263; https://doi.org/10.3390/bios14060263 (registeringDOI)
Submission received: 17 April 2024/Revised: 6 May 2024/Accepted: 14 May 2024/Published: 21 May 2024
(This article belongs to the Special Issue Emerging Applications of Label-Free Optical Biosensors)
Abstract
The spread of the FluA virus poses significant public health concerns worldwide. Fluorescent lateral flow immunoassay (LFIA) test strips have emerged as vital tools for the early detection and monitoring of influenza infections. However, existing quantitative virus-detection methods, particularly those utilizing smartphone-based sensing platforms, encounter accessibility challenges in resource-limited areas and among the elderly population. Despite their advantages in speed and portability, these platforms often lack user-friendliness for these demographics, impeding their widespread utilization. To address these challenges, this study proposes leveraging the optical pick-up unit (OPU) sourced from commercial optical drives as a readily available fluorescence excitation module for the quantitative detection of antibodies labeled with quantum-dot fluorescent microspheres. Additionally, we utilize miniaturized and high-performance optical components and 3D-printed parts, along with a customized control system, to develop an affordable point-of-care testing (POCT) device. Within the system, a stepping motor scans the test strip from the T-line to the C-line, enabling the calculation of the fluorescence-intensity ratio between the two lines. This simple yet effective design facilitates rapid and straightforward field or at-home testing for FluA. The proposed prototype platform demonstrates promising performance, achieving a limit of detection (LOD) of 2.91 ng/mL, a total detection time of no more than 15 min, and dimensions of 151 mm × 11.2 mm × 10.8 mm3. We believe that the proposed approach holds great potential for improving access to an accurate influenza diagnosis.
Keywords: lateral flow immunoassay; quantum-dot fluorescent microspheres; optical pick-up unit; FluA; point-of-care
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MDPI and ACS Style
Chen, X.; Huang, X.; Kanwal, S.; Wang, J.; Wen, J.; Zhang, D.A Portable Fluorescent Lateral Flow Immunoassay Platform for Rapid Detection of FluA. Biosensors 2024, 14, 263.https://doi.org/10.3390/bios14060263
AMA Style
Chen X, Huang X, Kanwal S, Wang J, Wen J, Zhang D.A Portable Fluorescent Lateral Flow Immunoassay Platform for Rapid Detection of FluA. Biosensors. 2024; 14(6):263.https://doi.org/10.3390/bios14060263
Chicago/Turabian Style
Chen, Xu, Xuhui Huang, Saima Kanwal, Jian Wang, Jing Wen, and Dawei Zhang.2024. "A Portable Fluorescent Lateral Flow Immunoassay Platform for Rapid Detection of FluA" Biosensors 14, no. 6: 263.https://doi.org/10.3390/bios14060263
Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.
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MDPI and ACS Style
Chen, X.; Huang, X.; Kanwal, S.; Wang, J.; Wen, J.; Zhang, D.A Portable Fluorescent Lateral Flow Immunoassay Platform for Rapid Detection of FluA. Biosensors 2024, 14, 263.https://doi.org/10.3390/bios14060263
AMA Style
Chen X, Huang X, Kanwal S, Wang J, Wen J, Zhang D.A Portable Fluorescent Lateral Flow Immunoassay Platform for Rapid Detection of FluA. Biosensors. 2024; 14(6):263.https://doi.org/10.3390/bios14060263
Chicago/Turabian Style
Chen, Xu, Xuhui Huang, Saima Kanwal, Jian Wang, Jing Wen, and Dawei Zhang.2024. "A Portable Fluorescent Lateral Flow Immunoassay Platform for Rapid Detection of FluA" Biosensors 14, no. 6: 263.https://doi.org/10.3390/bios14060263
Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.
Biosensors,EISSN 2079-6374,Published by MDPI
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